Recommended for the isolation and enumeration of coagulase positive staphylococci from food and clinical samples.
Ingredients:
Tryptone: 10 g/L
HM Peptone B#: 5 g/L
Yeast extract : 1 g/L
Glycine: 12 g/L
Sodium pyruvate: 10 g/L
Lithium chloride: 5 g/L
Agar: 20 g/L
Final pH ( at 25°C): 7.0±0.2
Directions:
Suspend 63.0 grams in 950 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 50 ml concentrated Egg Yolk Emulsion (FD045) and 3 ml sterile PTe 3.5% Selective Supplement (1 ml per vial) (FD047) or 50 ml Egg Yolk Tel Emulsion (100 ml per vial) (FD046). For additional selectivity, if desired add rehydrated contents of 1 vial of BP
S Selective Supplement (FD069). Alternatively 1 vial of FPT Inhibitor (FD195) may be used per 90 ml medium in place of Egg Yolk Tel Emulsion (100 ml per vial) (FD046) for identification of coagulase, positive Stapylococci. Mix well and pour into sterile Petri plates.
Quality Control:
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium: Basal medium: Yellow coloured clear to slightly opalescent gel. After addition of Egg Yolk Emulsion and Tellurite Emulsion: Yellow coloured opaque gel forms in Petri plates.
Reaction: Reaction of 6.3% w/v aqueous solution at 25°C. pH : 7.0±0.2
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