It is recommended for studying coagulase reaction in diagnosis of Staphylococci.
Ingredients - Concentration:
Coagulase plasma w/EDTA - 0.10 gm
Directions:
Rehydrate the contents of one vial aseptically with 3 ml sterile distilled water. Add 0.5 ml of rehydrated content of FD248A in a tube. To this add approximately 0.05 ml of overnight broth culture of test organisms or 2-3 pure colonies picked from a non inhibitory Agar plate. Mix gently & incubate at 37oC in an incubator or water bath for upto 4 hours. Observe for clot formation in the tube at regular intervals. Any degree of clotting within 4 hours is considered as positive results.
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