The HiMedia MBT213 Hi-Quik Tissue DNA Extraction Kit provides a fast, simple, and reliable silica-column-based method for isolating high-purity genomic DNA from a wide variety of animal tissues, mammalian cells, and clinical specimens. Engineered to eliminate tedious organic extractions and ethanol precipitation steps, this kit ensures maximum recovery of intact, high-molecular-weight DNA ready for downstream molecular biology applications.
Key Features & Benefits:
- High Yield & Purity: Delivers typical A260/A280 absorbance ratios of 1.7–1.9, ensuring minimal protein, RNA, and salt contamination.
- Rapid Spin-Column Workflow: Streamlined protocol allows complete tissue lysis, binding, washing, and elution in under 45 minutes.
- Broad Sample Compatibility: Optimized for rodent and mammalian solid tissues, tail clips, organ biopsies, culture cells, and paraffin-embedded (FFPE) sections.
- High Molecular Weight Integrity: Gentle chemistry preserves genomic integrity, yielding fragment sizes suitable for sensitive enzymatic assays.
- No Hazardous Organic Solvents: Completely eliminates the need for toxic phenol, chloroform, or isoamyl alcohol handling.
Applications:
Genomic DNA isolated using MBT213 is fully validated for demanding downstream workflows:
- Standard and real-time quantitative PCR (qPCR)
- Next-Generation Sequencing (NGS) library preparation
- Southern blotting and restriction enzyme digestion
- Genotyping, SNP analysis, and Sanger sequencing
- Forensic and comparative genomic studies
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