The HTC001E (often listed as HTC001E-5PR) is the HiPer® Ion Exchange Chromatography Teaching Kit produced by HiMedia Laboratories. It is a molecular biology teaching tool designed to demonstrate the principles of protein purification using ion exchange chromatography.
Core Educational Objectives
Protein Purification: Demonstrates the separation and purification of lysozyme from a complex biological source: chicken egg white.
Operational Principles: Teaches the concept of reversible interactions between charged molecules in a sample and oppositely charged groups on a stationary phase (matrix).
Enzymatic Estimation: Beyond purification, the kit allows students to estimate the enzymatic activity of the purified lysozyme.
Key Kit Components
The kit includes specific reagents and biological materials necessary for the experiment:
Matrix: CM-cellulose (Carboxymethyl cellulose).
Standards & Samples: Standard lysozyme and Micrococcus luteus culture (used for activity assays).
Buffers: Buffer A, Buffer B, and 1M Phosphate Buffer (pH 7.0)
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