Indole-3-butyric acid (IBA)
is a naturally occurring and synthetic plant hormone belonging to the auxin family. In plant tissue culture and horticulture, it is considered the "gold standard" for rooting, as it is more stable and effective than its counterpart, Indole-3-acetic acid (IAA).
Role in Plant Culture
IBA is primarily used to stimulate the initiation of adventitious roots in both micropropagation (tissue culture) and macropropagation (cuttings).
• Root Initiation: It triggers cell division in the pericycle or cambium, leading to the formation of root primordia.
• Callus Induction: In higher concentrations, it can be used in conjunction with cytokinins to induce callus formation from explants.
• Stability: IBA is less susceptible to degradation by light (photo-oxidation) or enzymatic breakdown (IAA-oxidases) within the plant, making it more reliable for long-term culture.
"Plant Culture Tested" Specification
When IBA is labeled as "Plant Culture Tested" it indicates that the chemical has undergone stringent quality control for laboratory use:
• Purity: Typically ≥98% TO 99 %
• Sterility and Solubility: Verified for ease of use in preparing sterile stock solutions.
• Biological Activity: Tested specifically for its ability to induce rooting in sensitive plant species without causing toxic side effects or unwanted morphology.
Preparation and Solubility
IBA is not soluble in water. To prepare a stock solution:
1. Dissolve: Dissolve the powder in a small volume of 95% Ethanol or 1N NaOH.
2. Dilute: Gradually add distilled water to reach the final volume.
3. Sterilization: It is heat-stable and can be autoclaved with the culture medium (e.g., Murashige and Skoog Medium), although filter-sterilization is sometimes preferred for precise research to avoid any thermal degradation.
Customer Reviews