Recommended for isolation and identification of pathogenic Staphylococci and Bacillus species.
Ingredients:
Peptone: 10 g/L
HM Extract (equivalent to Meat extract): 1 g/L
D-Mannitol: 10 g/L
Sodium chloride: 10 g/L
Phenol Red: 0.025 g/L
Agar: 15 g/L
Final pH ( at 25°C): 7.1±0.2
Directions:
Suspend 23.01 grams in 450 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add rehydrated contents of one vial of sterile PolyB Selective Supplement (FD003) solution to a final concentration of 100 units per ml and 50 ml sterile Egg Yolk Emulsion (FD045). Mix well and pour into sterile Petri plates.
Quality Control:
Appearance: Light yellow to light pink homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Basal medium :Red coloured clear to slightly opalescent gel. After Addition of Egg Yolk Emulsion (FD045) : Light orange coloured opaque gel forms in Petri plates
Reaction: Reaction of 4.6% w/v aqueous solution at 25°C. pH : 7.1±0.2
Customer Reviews