Middlebrook 7H9 Broth Base, is a specialized liquid growth medium used in clinical and research microbiology. It is carefully engineered for the cultivation, propagation, and antimicrobial sensitivity testing of Mycobacterium tuberculosis and other fastidious Mycobacterium species.
Intended Use
Recommended as a general purpose medium used for cultivation and maintenance of Salmonella Typhi.
Composition
Ingredients Gms / Litre
Tryptone 17.000
Soya peptone 3.000
Sodium chloride 5.000
Dextrose (Glucose) 2.500
Dipotassium hydrogen phosphate 2.500
Agar 15.000
Final pH ( at 25°C): 7.3±0.2
**Formula adjusted, standardized to suit performance parameters
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