HiMedia Urea Agar Base (Christensen) is a dehydrated culture medium widely used in microbiology to detect urease production in microorganisms. It is primarily employed to differentiate rapid urease-positive enteric bacteria like Proteus species from other non-utilizing Gram-negative bacilli like Salmonella and Shigella.
Composition
Ingredients g/L
Peptone 1.000
Dextrose (Glucose) 1.000
Sodium chloride 5.000
Disodium hydrogen phosphate 1.200
Potassium dihydrogen phosphate 0.800
Phenol red 0.012
Agar 15.000
Final pH (at 25°C) 6.8±0.2
Formula adjusted, standardized to suit performance parameters
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