The HiPer® Thin Layer Chromatography (TLC) Teaching Kit (SKU: HTC003-10PR) is an educational laboratory resource from HiMedia Laboratories designed to teach the fundamental principles of chromatography. This specific 10-preparation kit enables students to separate and identify individual amino acids from a complex mixture.
Core Objectives
Separation Technique: Demonstrates how biomolecules distribute between a stationary phase (silica-coated plates) and a mobile phase (solvent).
Identification: Teaches students how to identify unknown amino acids by calculating and comparing their Retardation Factor (Rf) values.
Visualization: Illustrates the chemical reaction between Ninhydrin reagent and amino acids, which produces characteristic colored spots (typically purple/violet).
Kit Contents (10 Preparations)
The kit includes reagents and materials sufficient for 10 experiments:
Standard Amino Acid Samples: Arginine, Glutamine, Glycine, Histidine, Isoleucine, Proline, Threonine, Tryptophan, and Valine.
Test Sample: An unknown mixture for student identification.
Hardware: 10 TLC plates (silica-coated).
Chemicals: TLC Solvent (mobile phase) and Developing Reagent (Ninhydrin).
Storage and Shelf Life
Stability: The kit is stable for 6 months from the date of manufacture.
Refrigerated (2–8°C): Amino acid standards and the test sample.
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