Tryptone Soya Agar with Lecithin and Polysorbate 80 is used in RODAC (Replicate Organism Detection and Counting) plates (1) for the detection and enumeration of microorganisms present on surfaces of sanitary importances (2,3). Tryptone and Soya peptone provide nitrogenous compounds and other nutrients essential for microbial replication. Lecithin and polysorbate 80 (Tween 80) are neutralizers reported to inactivate residual disinfectants from where the sample is collected (4). Lecithin neutralizes quaternary ammonium compounds and polysorbate 80 neutralizes phenolic disinfectants, hexachlorophene, formalin and with lecithin ethanol (5).
Composition**
Ingredients Gms / Litre
Tryptone 15.000
Soya Peptone 5.000
Sodium chloride 5.000
Lecithin 0.700
Polysorbate 80 (Tween 80) 5.000
Agar 15.000
Final pH (at 25°C) 7.3±0.2
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